Comparative Study of Various Culture Media for Selecting the Enhancement of Mycelial Proliferation of Pleurotus ostreatus

Amita Gaurav Dimri*, Dushyant Singh, Sumant Kumar Nayak, Binu Bhat and Mukul Das

The cultivation of oyster mushroom (Pleurotus ostreatus) mycelium is a potential challenge in agro-biotechnological and food industries, due to the strain-selectivity, specificity, and productivity of growth pattern. The purpose of the study was to examine the effects of seven distinct culture media (PDA, WEA, CMA, CYGA, SDA, TSA, and PCA) on the mycelium growth of P. ostreatus. They were assessed in in-vitro aseptic conditions in a randomized design with five replicates. The radial mycelium development of P. ostreatus was monitored and documented every 48 hours for eight days in all seven media.

The findings of the experiment showed that after completion of the second day of incubation, there was an insignificant difference (p<0.05) in mycelial growth of P. ostreatus between various treatments used. The impact of various media on mycelial multiplication of the target oyster mushroom was significantly enhanced during the fourth day of inoculation. Additionally, several noticeable significant differences (p < 0.05) were found in potato dextrose agar, wheat extract agar, and Sabouraud dextrose agar in the form of increased and heavy mycelial growth, as 4.53, 4.24, and 4.26 centimeters, respectively, whereas comparatively the least mycelial growth was observed in CYGA, PCA, and CMA. After the eighth day of incubation, the data revealed that, according to the mycelial degree of growth with greater adaptive ability to grow on culture media, can be presented in the following order: PDA≈ WEA> SDA > TSA > CYGA > PCA > CMA. The capacity of the study is also to modify the culture technique for optimized growth of P. ostreatus for the method’s greatest advantage.

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Published on: Sep 25, 2025 Pages: 25-29

Full Text PDF Full Text HTML DOI: 10.17352/ojb.000030
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