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Annals of Systems Biology

    Abstract

    Open Access Opinion Article ID: ASB-7-123

    A Rapid Fluorescent in vitro Assay Suitable for Studying the Kinetics of O6-Alkylguanine Lesion Progression to DNA Inter-Strand Cross-Links and the Kinetics of the Primary Lesion’s Repair

    Philip G Penketh*

    An assay is described that permits the study of the kinetics of DNA cross-link precursor formation, the kinetics of cross-formation, and the kinetics of the repair of these DNA cross-link precursor lesions, at physiological pH values. Due to the relatively rapid nature of these processes existing assays are not suited to the study of these processes. Data obtained using this assay can be used to optimize the design of certain classes of anticancer drugs.

    Keywords:

    Published on: Nov 15, 2024 Pages: 51-53

    Full Text PDF Full Text HTML DOI: 10.17352/asb.000023
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    Laval University, Canada
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    Miklas Scholz
    Lund University, Sweden
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    William CS Cho
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    Jinde Cao
    Southeastern University, China
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    Francesco Marotta
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    Thomas F. George
    University of Missouri - St. Louis, USA
    Open Journal of Analytical and Bioanalytical Chemistry
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    Private Compnay- Biological Mimetics, Inc., USA
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    Giampiero Capobianco
    Sassari University, Italy
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