https://www.biolscigroup.us/journals/global-journal-of-biotechnology-and-biomaterial-science A Peertechz Open Access Journal en-us 31 Dec, 2025 https://www.biolscigroup.us/articles/GJBBS-11-123.php Tuberous sclerosis complex (TSC) is a rare genetic disorder marked by benign tumor formation in multiple organs, frequently involving the heart and central nervous system (CNS). We present a case study of a premature infant with genetically confirmed TSC and coexisting cardiac rhabdomyomas (CRs) and subependymal giant-cell astrocytomas (SEGAs). The patient was treated with oral sirolimus starting at 3 months of age. Cardiac lesions exhibited complete resolution within 6 months of therapy, as evidenced by echocardiography and normal myocardial strain. CNS imaging at 12 months showed partial SEGA regression. Treatment was well tolerated, with no adverse events reported. This case illustrates the potential of early sirolimus therapy to accelerate tumor regression in both cardiac and CNS lesions, highlighting its value in the conservative management of TSC in infancy. 26 Jul, 2024 https://www.biolscigroup.us/articles/GJBBS-10-122.php Understanding the response of materials to various loads is crucial for fabricating resilient components capable of withstanding anticipated stresses. This experimental study addresses the limited information on the tensile behavior of silicon bronze by investigating the stress-strain characteristics of Cu-4wt%Si alloys modified with niobium nanopowder. The surface morphology of the fabricated alloys was analyzed using a Scanning Electron Microscope (SEM). SEM analysis of the Cu-4wt%Si alloy revealed columnar grains sparsely distributed in the copper matrix, contributing to low strain-to-fracture behavior. Doping the alloy with niobium nanopowder resulted in notable grain modification, increased grain boundary density, and improved grain distribution, correlating with higher strain-to-fracture values. Notably, the Cu-4wt%Si-1.1wt%Nb alloy exhibited significant changes in percentage elongation, ultimate tensile strength, yield strength, fracture strength, and tensile toughness. Furthermore, niobium nanopowder additions increase the hardness and electrical conductivity values, peaking at 305 HV and 54.9 %IACS with 1.1 wt% Nb. 12 Jul, 2024 https://www.biolscigroup.us/articles/GJBBS-10-121.php Rice, as a staple food crop, faces various difficulties from biotic burdens like sicknesses, bugs, and weeds. Customary raising strategies have shown a halfway outcome in creating rice arrangements with a thorough battle against numerous biotic burdens. The superbiotic elements that lessen rice efficiency are bacterial curse (BB) and organism-impact diseases. The potential for yield of the rice varieties must be developed by including genes for biotic stress resistance if yield improvements in rice are to be sustained. This research paper aims to explore innovative methods of gene pyramiding to upgrade biotic stress, and resilience in rice varieties. We examine the standards and headways in quality pyramiding procedures, including sub-atomic markers, and determination on marker-helped and genome-altering apparatuses, to stack numerous opposition qualities proficiently. By utilizing these methods, we propose a clever methodology for raising rice assortments with upgraded biotic pressure patience. In this review, two principal BB (Xa21 and xa13) and two critical impact obstruction qualities (Pi54 and Pi1) were all the while brought into Tellahamsa with the use of the marker-helped backcross rearing (MABB) system. The paper underlines the expected advantages, challenges, and moral contemplations related to quality pyramiding, and talks about future exploration bearings to speed up the organization of versatile rice assortments. 16 Feb, 2024 https://www.biolscigroup.us/articles/GJBBS-10-120.php Medicine has changed rarely in history as rapidly as it is today. Constantly new methods are being introduced that can improve health outcomes. Six such developments are presented in this article, that is, Artificial Intelligence (AI) in diagnosis and treatment, 3D-printed organs, tele-surgery, nano-medicine, CRISPR technology, and quantum teleportation. Thus, with these developments, several problems in medicine can be solved to the benefit of patients. However, it also increases the responsibility of users to apply the methods in accordance with ethical principles. 10 Jun, 2023 https://www.biolscigroup.us/articles/GJBBS-9-119.php Traditional Chinese Medicine (TCM) is widely used in health preservation and non-radical treatment because of its relatively gentle therapeutic effect, which is reflected in many products. Especially in atomization therapy, TCM shows its strong potential. This experiment focused on analyzing the extract of green tea (tea polyphenols) in a nebulizer, which is very similar to e-cigarettes. After intravenous injection of relevant drugs into small experimental pigs, the results of blood tests and the HE staining of organ sections showed no abnormalities in the experimental group. Since the dose of intravenous injection is much larger than that of nebulized inhalation, the safety of the product was verified by the experiment. The relevant analysis results obtained in this experiment will be used for comparison with subsequent, longer-term (more than 3 months) injection experiments. 16 Mar, 2023 https://www.biolscigroup.us/articles/GJBBS-9-118.php Purpose: The aim of this study was to evaluate the safety and toxicity of intravenous use of White Tea Extract Liquid (WT) in rabbits through routine blood tests and biochemical and histopathological means. Design and methods: This study used the WT product produced by the company and the New Zealand rabbits at 2.0~2.5 kg of weight. Rabbits were divided into two groups: Control and WT groups. In the WT group, the diluted WT injection volume needed to be injected was calculated according to their body weight on days 1, 4, 7 and 11...... for 4 weeks respectively. During the1, 2 and 4 weeks of the experiment, blood routine, blood biochemistry and the histopathological sections of the heart, spleen, lung, trachea, kidney, bladder, uterus, testicle, stomach, liver, small intestine, big intestine were evaluated in the study. Findings: By comparison, the blood routine test did not deviate from the normal ranges provided by the diagnostic laboratory. The blood biochemical indexes of all experimental rabbits are within a reasonable range and did not detect clinically significant abnormalities between treatment and control groups. The WT had no obvious inflammatory change in the whole tissue and no harmful effect on the organs of various systems of the rabbits’ system for 4 weeks. Conclusions: After intravenous injection for 4 weeks in rabbits, white tea herb atomization liquid did not produce obvious toxic effects on the animals. 14 Jun, 2022 https://www.biolscigroup.us/articles/GJBBS-8-117.php The main aim of this research is to experimentally investigate the two coupled identical ST500 gamma-type Stirling engines and convert thermal energy to cooling energy. Using a new structure and two coupled Stirling engines at different temperatures and pressures and the use of biomass fuel within the 4 -8 bar average pressure range of the first engine heat source, the 1-4 bar average pressure range of the second engine heat sink, and Stirling heat engine temperature range of 480-580 ºC, the effective cooling is obtained in the cooling engine. In doing tests, attempts were made to reach lower than 9 percent error results in different parts of the engine, including insulation, fluid leakage, belt loss, and measurement devices. According to the obtained results, 8 bars increase in the average pressure range of the gas in the first engine heat source, a 1 bar reduction in the average pressure range of the gas in the second engine heat sink, the increased temperature of the heat source up to 580ºC, and the use of the light operating fluid such as helium will affect the generation of cooling up to-16ºC. 25 May, 2022 https://www.biolscigroup.us/articles/GJBBS-8-116.php Skeletal muscle is an important part of the human body. Most glucose metabolism is accomplished by skeletal muscle through insulin mediation. Skeletal muscle metabolism disorder can affect glucose metabolic homeostasis and insulin sensitivity of the body, and diabetic muscular Hypoplasia is a secondary lesion of muscle tissue caused by diabetes. In recent years, it has been found that in addition to mainstream western medicine and traditional Chinese and Western medicine combined treatment programs, natural products, and nutritional supplements also play an important role in the prevention and treatment of diabetic muscular Hypoplasia. Therefore, this paper will discuss the definition and pathogenesis of diabetic muscular Hypoplasia, as well as the prevention and treatment mechanism of some natural products and nutritional supplements, to provide more theoretical reference for non-drug targeted therapy of diabetic muscular Hypoplasia. 12 Jan, 2021 https://www.biolscigroup.us/articles/GJBBS-7-115.php 24 Nov, 2020 https://www.biolscigroup.us/articles/GJBBS-6-114.php Allium sativum (garlic) is one of the most famous plant rich in Sulphur products which are so beneficial in bio-gardening and agriculture if applied in right ratios as natural fertilizer to the soil poor in organic matter (under 2%) or applied as natural fungicide &pesticide. It like many other important folk plants needs further investigations and revising for its products’ cytogenecity and mutagenicity. This study was designed to highlight the mode of action of fresh garlic water extract against two other garlic products (powder and tablets) dissolved in water on faba bean plant, as an ideal model system for both cytogenetic and biochemical investigations. Tomix tablets in water (two and three tablets/L), garlic powder (400 and 600 mg/L) and aqueous extract of smashed fresh garlic cloves (4500 and 7110 mg/L) were examined for their effect on plant mitosis and protein profile after 24 and 48hr of treatments. The obtained results showed that; treatment with each form of the three tested garlic products induced concentration dependent reduction in the mitotic index, and affected notable change in the mitotic phase’ frequencies. Its effect on chromatin material is much greater than its effect on spindle formation or chromosomes mobility in cytoplasm. It also affected the proteins profile of the treated seedlings using SDS-PAGE. Conclusion: Treatment with fresh garlic and with garlic powder shows concentration dependent cytogenecity and mutagenecity. Prolongation of treatment time to 48hr increases the clastogenecity risk. Recommendation: the usage of fresh Garlic extract as a fungi/ pesticide in agriculture should be with caution, as it show some mutagenicity and cytogenicity. 10 Jun, 2020 https://www.biolscigroup.us/articles/GJBBS-6-113.php Parkinson’s disease, caused by mutations in the Parkin that leads to loss of neuron is the second most widespread neurodegenerative disorder in the world. 28 May, 2020 https://www.biolscigroup.us/articles/GJBBS-6-112.php Background: T. spiralis establishes an intimate contact with the host erythrocytes during the newborn larvae migration through the bloodstream to their encystment in the muscle. Objective: In the present work we study the alterations in the mechanical and aggregation properties of red blood cells produced in vitro by newborn larvae at low concentrations (100, 250, 500, and 1000 larvae/mL). Methods: The study was performed by incubating human erythrocytes with an equal volume of different concentrations of newborn larvae for 30 minutes, with controlled agitation. To evaluate the erythrocyte alterations by the action of the larvae, the Erythrocyte Rheometer, the Optical Chip Aggregometer, and the Digital Image Analysis were used. 16 May, 2020 https://www.biolscigroup.us/articles/GJBBS-6-111.php This work studied the use of several plant extracts as probable medications in the treatment of diabetes. In laboratory rats, the diabetes model was developed by injection of Streptozotocin (STZ) in a dose of 40 mg/kg of body weight. The animals, in which the blood glucose level increased 4-5 times, were considered as diabetic. The diabetic animals were treated by ethanol extracts of plants traditionally used in folk medicine and/or as food in Armenian cuisine. During four weeks, the STZ-induced diabetic rats were administered orally three times in a week with the ethanol extracts from sorrel leaves (SL, Rumex Confertus), pellicles of walnut kernel (WP, Juglans regia), grape leaves (GL, Vitis vinifera) and rose petals (RP, Rosa damascena). At the end of the experiment, certain decrease of blood glucose level was registered in these groups. An oxidative stress marker, malondialdehyde, which increased in the blood of the STZ-induced diabetic animals by 38%, decreased in the SL and WP groups to the level of the intact animals. The histo­pathological examination of the pancreas, kidney and liver sections from the diabetic animals, fed with the extracts from SL, RP and WP, revealed positive changes compared with the STZ-control animals. The obtained results manifest the benefit of using the studied herbal extracts in the STZ-induced diabetes model. 10 Aug, 2018 https://www.biolscigroup.us/articles/GJBBS-4-110.php Protein purification and crystallization problems have been noticed for many years. The strategies developed for protein solubility and crystallization have improved the protein production and provide high-resolution crystals for structural studies. The protein solubility is achieved by the Site-directed mutagenesis that generates the hydrophobic to hydrophilic mutations. However, the purity and rate of crystallization success still needs to be improved. 14 Apr, 2017 https://www.biolscigroup.us/articles/GJBBS-3-109.php Molecularly imprinted polymer (MIP) nanoparticles, commonly referred to as ‘plastic antibodies’ or synthetic receptors, are polymeric materials with strong affi nity and selectivity for a particular chemical target. 30 Sep, 2016 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-2-108.php The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic strength, pH, temperature, and organism source of macromolecules, reducing or oxidizing environment, additives, ligands, presence of substrates, inhibitors, coenzymes, metal ions and rate of equilibration. The aim of this paper to give very helpful advice for crystallization. 16 Sep, 2016 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-2-107.php Objective: The systematic objective of this paper is to restore flexion in vertebral bone and to nourish the nucleus pulposus through the approach of biomaterial and regenerative medicine. Method: Studies were made on the treatment of lumbar herniated disc in CINAHIL, PubMed, EMBASE, Cochrane Central Register of Controlled Trials (CENTRAL) and Cochrane Database of Systematic Reviews(CDSR). This paper provides methods for restoration of flexion in the bone. A biomaterial scaffold made of poly-β-hydroxybutyrate (PHB) consisting of extracellular matrix (ECM) proteins which is synthesized in vitro through regenerative medicine is replaced in the place of degenerated disc. The scaffold model is designed in CAD and then printed in LAB. The metabolic functions of the disc are performed by the scaffold. 28 Apr, 2016 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-2-106.php One of the biggest issues in today’s healthcare industry is to find a very fast yet effective diagnostic platform, which is suitable for our busy lifestyle without compromising the detection efficiency. The major requirements of an efficient diagnostic platform can be summarized as minimum sample requirement, least reagent requirement, having options of multiple tests in a single platform, high through put analysis and last but never the least a non-expensive sample run. The largest contributor of the bio-assay industry is protein based chromogenic bio-assays, which depends on strong antigen-antibody interaction and high emissive properties of reporter dye molecule attached to the antigen. 22 Feb, 2016 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-2-105.php Background and Aim: Drug-induced nephrotoxicity is a renal dyfunction that arises as a result of exposure to nephrotoxic drugs. Anti-tuberculosis therapy can cause nephrotoxicity and permanent kidney damage. The current study was designed to evaluate the possible protective effects of Ruta graveolens L. leaves extract against isoniazid/rifampicin-induced nephrotoxicity in rats.. 27 Jan, 2016 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-2-104.php Bioactive ceramic materials have been under research as bone substitute for several decades. To repair the high-load bearing bones, mainly cortical bones, there is a need for the substitute to possess comparable mechanical strength to cortical bone, of which the compressive strength ranges between 100 and 230 MPa. Two prevailing bone repairing material, β-tricalcium phosphate (β-TCP, β-Ca3(PO4)2) and hydroxyapatite (HAp: Ca10(PO4)6(OH)2) have been widely researched and sintered into dense blocks to meet the mechanical requirements. α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2), a high temperature polymorph of β-TCP, received relatively less attention and α-TCP dense sintered blocks have not been reported yet. In this research, we fabricated α-TCP dense blocks by sintering under various temperatures (1150-1400 °C) and the highest compressive strength was around 230 MPa. 06 Jul, 2015 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-1-103.php MicroRNAs (miRNAs) are a family of short non-coding RNA molecules which contain about 18-24 nucleotides. miRNAs play significant regulatory roles in biological processes (cell proliferation, differentiation, survival and motility). miRNAs are essential regulators of coding genes at the post-transcriptional level. The biogenesis of miRNAs is composed of complex processes, and human genome contains approximately two thousand miRNAs (http://www.mirbase.org) [11. Tutar L, Tutar E, Tutar Y (2014) MicroRNAs and cancer; an overview. Curr Pharm Biotechnol 15: 430-437.,22. Calin GA, Croce CM (2006) MicroRNA signatures in human cancers. Nat Rev Cancer 11: 857-866.]. Expression patterns of miRNAs have been found to have links with pathogenesis of many diseases including neurodegenerative diseases, diabetes, cardiovascular diseases, and particularly cancer. miRNAs have been identified as a critical biomarker for treatment, diagnosis, and progression of cancer. 25 May, 2015 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-1-102.php Most aquatic and health management authorities need assessing the potential genotoxicity of contaminated effluent to avoid the effects of a lot chemical agents found as environmental contaminants. Previous studies report the presence of some pesticides and heavy metals in waters of Santiago River and suggest a genetic risk for residents, who lived nearby this river, so it is important determine its mutagenic risk. The objective of this study was to use of comet assay in human lymphocytes as a molecular biomarker of genetic damage in residents who lived nearby the Santiago River, Mexico, in 2012 and monitoring simultaneous of genotoxicity of these waters in unexposed persons. The genotoxic activity of water samples taken from the Santiago River was evaluated in human lymphocytes from persons unexposed and the evaluation of genetic damage were studied in people living near the river. 17 Apr, 2015 https://www.biolscigroup.us/Biotechnology-Biomaterial-Science/GJBBS-1-101.php This study found that Aloe vera mucilage administration significantly improved biological parameters including lipids composition and oxidative stress in type 2 diabetic rats. Because the mRNA levels of lipogenic genes were significantly down-regulated (P<0.01) by the intervention of A. vera mucilage, this improvement of these biological parameters may be caused, at least in part, by the regulation of hepatic genes that are involved in lipids metabolism, thus resulting in an increase of cholesterol clearance and β-oxidation as well as a reduction of hepatic lipogenesis. The results in this study might provide a further beneficial effect of A. vera mucilage in the possible management of dyslipidemia-related diseases.